Western blot analysis of PIAS3 over-expressed 293 cell line, cotransfected with PIAS3 Validated Chimera RNAi ( Cat # H00010401-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIAS3 monoclonal antibody (M03), clone 4F12 (Cat # H00010401-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq