PEMT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PEMT full-length
Host
Human
Theoretical MW (kDa)
26.07
Interspecies Antigen Sequence
Mouse (80); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PEMT antibody (H00010400-B02) by Western Blots.
SDS-PAGE Gel
PEMT transfected lysate.
Western Blot
Lane 1: PEMT transfected lysate ( 26.07 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PEMT
Entrez GeneID
10400GeneBank Accession#
NM_148172.1Protein Accession#
NP_680477.1Gene Name
PEMT
Gene Alias
MGC2483, PEAMT, PEMPT, PEMT2, PNMT
Gene Description
phosphatidylethanolamine N-methyltransferase
Omim ID
602391Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000065536|OTTHUMP00000065537
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Interactome
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