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Last updated: 2017/11/19

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PEMT 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00010400-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PEMT full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 26.07
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PEMT antibody (H00010400-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00010400-T01
    PEMT transfected lysate
    Western Blot
    QC Testing of H00010400-T01
    Lane 1: PEMT transfected lysate ( 26.07 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • PEMT
  • Gene Alias:
  • MGC2483,PEAMT,PEMPT,PEMT2,PNMT
  • Gene Description:
  • phosphatidylethanolamine N-methyltransferase
  • Gene Summary:
  • This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000065536,OTTHUMP00000065537
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