HMG20B monoclonal antibody (M01), clone 1F6
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More Files
- More Functions
Western Blot (Cell lysate)
HMG20B monoclonal antibody (M01), clone IF6 Western Blot analysis of HMG20B expression in HeLa ( Cat # L013V1 ).
Western Blot (Cell lysate)
Western blot analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HMG20B on formalin-fixed paraffin-embedded human lung cancer. [antibody concentration 1.8 ug/ml]
Immunofluorescence
Immunofluorescence of monoclonal antibody to HMG20B on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HMG20B.
Immunogen
HMG20B (NP_006330, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HMG20B monoclonal antibody (M01), clone IF6 Western Blot analysis of HMG20B expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
Western blot analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody.Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HMG20B on formalin-fixed paraffin-embedded human lung cancer. [antibody concentration 1.8 ug/ml]ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HMG20B on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HMG20B
Entrez GeneID
10362GeneBank Accession#
NM_006339Protein Accession#
NP_006330Gene Name
HMG20B
Gene Alias
BRAF25, BRAF35, FLJ26127, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857
Gene Description
high-mobility group 20B
Omim ID
605535Gene Ontology
HyperlinkGene Summary
member 1-related|Sox-like transcriptional factor
Other Designations
HMG box domain containing 2|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related|Sox-like transcriptional factor
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Interactome
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Publication Reference
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The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.
Zanchetta ME, Napolitano LMR, Maddalo D, Meroni G.
Biochimica et Biophysica Acta 2017 Jul; 1864(10):1844.
Application：WB-Tr, Human, HEK 293T, HeLa cells.
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Control of neuronal differentiation by sumoylation of BRAF35, a subunit of the LSD1-CoREST histone demethylase complex.
Ceballos-Chávez M, Rivero S, García-Gutiérrez P, Rodríguez-Paredes M, García-Domínguez M, Bhattacharya S, Reyes JC.
PNAS 2012 May; 109(21):8085.
Application：WB, Human, HEK 293 cells.
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The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.
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