NPM2 monoclonal antibody (M03), clone 5E9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant NPM2.
Immunogen
NPM2 (NP_877724.1, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNLSSASSTEEKAVTTVLWGCELSQERRTWTFRPQLEGKQSCRLLLHTICLGEKAKEEMHRVEILPPANQEDKKMQPVTIASLQASVLPMVSMVGVQLSPPVTFQLRAGSGPVFLSGQERYEASDLTWEEEEEEEGEEEEEEEEDDEDEDADISLEEQSPVKQVKRLVPQKQASVAKKKKLEKEEEEIRASVRDKSPVKKAKATARAKKPGFKK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (65); Rat (62)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (50.6 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NPM2 expression in transfected 293T cell line by NPM2 monoclonal antibody (M03), clone 5E9.
Lane 1: NPM2 transfected lysate (Predicted MW: 24.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NPM2 is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NPM2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NPM2
Entrez GeneID
10361GeneBank Accession#
NM_182795.1Protein Accession#
NP_877724.1Gene Name
NPM2
Gene Alias
MGC78655
Gene Description
nucleophosmin/nucleoplasmin, 2
Omim ID
608073Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000161773|nucleoplasmin 2
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