DNAJA2 monoclonal antibody (M01), clone 2A11-F2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant DNAJA2.
Immunogen
DNAJA2 (AAH13044, 1 a.a. ~ 412 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDRLSELEDLLPSRPEVPNIIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (71.06 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DNAJA2 monoclonal antibody (M01), clone 2A11-F2 Western Blot analysis of DNAJA2 expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DNAJA2 is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DNAJA2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — DNAJA2
Entrez GeneID
10294GeneBank Accession#
BC013044Protein Accession#
AAH13044Gene Name
DNAJA2
Gene Alias
CPR3, DJ3, DJA2, DNAJ, DNJ3, HIRIP4, PRO3015, RDJ2
Gene Description
DnaJ (Hsp40) homolog, subfamily A, member 2
Omim ID
611322Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq
Other Designations
DnaJ subfamily A member 2|HIRA interacting protein 4|cell cycle progression 3 protein
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Interactome
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Publication Reference
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Cell line specific modulation of extracellular aβ42 by Hsp40.
Carnini A, Scott LO, Ahrendt E, Proft J, Winkfein RJ, Kim SW, Colicos MA, Braun JE.
PLoS One 2012 May; 7(5):e37755.
Application:WB-Ce, Mouse, CAD cells.
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Quercetin targets cysteine string protein (CSPalpha) and impairs synaptic transmission.
Xu F, Proft J, Gibbs S, Winkfein B, Johnson JN, Syed N, Braun JE.
PLoS One 2010 Jun; 5(6):e11045.
Application:WB-Ce, Mouse, CAD mouse neuroblastoma cells.
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RDJ2 (DNAJA2) chaperones neural G protein signaling pathways.
Rosales-Hernandez A, Beck KE, Zhao X, Braun AP, Braun JE.
Cell Stress & Chaperones 2008 Jul; 14(1):71.
Application:WB, Human, Rat, Human embryonic kidney tsa-201 (HEK) cells, Rat brain.
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Cell line specific modulation of extracellular aβ42 by Hsp40.
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