SPRY2 monoclonal antibody (M01), clone 1E10
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More Files
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Western Blot (Cell lysate)
SPRY2 monoclonal antibody (M01), clone 1E10 Western Blot analysis of SPRY2 expression in C32 ( Cat # L002V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10.
Lane 1: SPRY2 transfected lysate(34.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25 ug/ml]
Western Blot detection against Immunogen (60.39 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SPRY2.
Immunogen
SPRY2 (AAH15745.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (95)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (60.39 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SPRY2 monoclonal antibody (M01), clone 1E10 Western Blot analysis of SPRY2 expression in C32 ( Cat # L002V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10.
Lane 1: SPRY2 transfected lysate(34.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25 ug/ml] -
Gene Info — SPRY2
Entrez GeneID
10253GeneBank Accession#
BC015745Protein Accession#
AAH15745.1Gene Name
SPRY2
Gene Alias
MGC23039, hSPRY2
Gene Description
sprouty homolog 2 (Drosophila)
Omim ID
602466Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq
Other Designations
OTTHUMP00000018543|OTTHUMP00000018544|sprouty 2
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Interactome
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Pathway
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Disease
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Publication Reference
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SPROUTY2 is a β-catenin and FOXO3a target gene indicative of poor prognosis in colon cancer.
Ordonez-Moran P, Irmisch A, Barbachano A, Chicote I, Tenbaum S, Landolfi S, Tabernero J, Huelsken J, Munoz A, Palmer HG.
Oncogene 2014 Apr; 33(15):1975.
Application：IF, IHC-P, WB, Human, Mouse, DLD1Fb cells, Human colon cancer, Small intestine.
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Initial report on differential expression of sprouty proteins 1 and 2 in human epithelial ovarian cancer cell lines.
Moghaddam SM, Amini A, Wei AQ, Pourgholami MH, Morris DL.
Journal of Oncology 2012 Nov; 2012:373826.
Application：IF, IHC-P, WB, Human, 1A9, A2780, CAOV-3, HOSEpiC, IGROV-1, OV-90, OVCAR-3, SKOV-3 cells, Human epithelial ovarian cancer.
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SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity.
Barbachano A, Ordonez-Moran P, Garcia JM, Sanchez A, Pereira F, Larriba MJ, Martinez N, Hernandez J, Landolfi S, Bonilla F, Palmer HG, Rojas JM, Munoz A.
Oncogene 2010 Aug; 29(34):4800.
Application：IF, IHC, WB-Ce, WB-Tr, Human, Mouse, DLD-1, HT-29, KM12SM, SW480, SW1417 cells, Mouse xenografts tumors.
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SPROUTY2 is a β-catenin and FOXO3a target gene indicative of poor prognosis in colon cancer.
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