SPRY1 monoclonal antibody (M01), clone 3H4
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More Files
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Western Blot (Cell lysate)
SPRY1 monoclonal antibody (M01), clone 3H4 Western Blot analysis of SPRY1 expression in HepG2 ( Cat # L019V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M01), clone 3H4.
Lane 1: SPRY1 transfected lysate(35.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SPRY1 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPRY1 is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SPRY1.
Immunogen
SPRY1 (NP_005832, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SPRY1 monoclonal antibody (M01), clone 3H4 Western Blot analysis of SPRY1 expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M01), clone 3H4.
Lane 1: SPRY1 transfected lysate(35.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SPRY1 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPRY1 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — SPRY1
Entrez GeneID
10252GeneBank Accession#
NM_005841Protein Accession#
NP_005832Gene Name
SPRY1
Gene Alias
hSPRY1
Gene Description
sprouty homolog 1, antagonist of FGF signaling (Drosophila)
Omim ID
602465Gene Ontology
HyperlinkGene Summary
O
Other Designations
sprouty homolog 1, antagonist of FGF signaling|sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
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Interactome
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Pathway
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Disease
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Publication Reference
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Targeted methylation sequencing reveals dysregulated Wnt signaling in Parkinson disease.
Zhang L, Deng J, Pan Q, Zhan Y, Fan J, Zhang K, Zhang Z.
Journal of Genetics and Genomics = Yi Chuan Xue Bao 2016 May; 43(10):587.
Application：IHC-P, Human, Human brain from patients with Parkinson disease.
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Human Sprouty1 suppresses growth, migration, and invasion in human breast cancer cells.
Mekkawy AH, Pourgholami MH, Morris DL.
Tumour Biology 2014 May; 35(5):5037.
Application：ICC, WB-Tr, Human, MDA-MB-231, T47D cells.
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Initial report on differential expression of sprouty proteins 1 and 2 in human epithelial ovarian cancer cell lines.
Moghaddam SM, Amini A, Wei AQ, Pourgholami MH, Morris DL.
Journal of Oncology 2012 Nov; 2012:373826.
Application：IF, IHC-P, WB, Human, 1A9, A2780, CAOV-3, HOSEpiC, IGROV-1, OV-90, OVCAR-3, SKOV-3 cells, Human epithelial ovarian cancer.
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Targeted methylation sequencing reveals dysregulated Wnt signaling in Parkinson disease.
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