MSLN 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MSLN transfected lysate.
Western Blot
Lane 1: MSLN transfected lysate ( 68.42 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MSLN full-length
Host
Human
Theoretical MW (kDa)
68.42
Interspecies Antigen Sequence
Mouse (58); Rat (57)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MSLN antibody (H00010232-B01) by Western Blots.
SDS-PAGE Gel
MSLN transfected lysate.
Western Blot
Lane 1: MSLN transfected lysate ( 68.42 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MSLN
Entrez GeneID
10232GeneBank Accession#
BC003512.1Protein Accession#
AAH03512.1Gene Name
MSLN
Gene Alias
CAK1, MPF, SMR
Gene Description
mesothelin
Omim ID
601051Gene Ontology
HyperlinkGene Summary
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
megakaryocyte potentiating factor
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Interactome
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Disease
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