MSLN monoclonal antibody (M02), clone 1A10
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More Files
- More Functions
Immunoprecipitation
Immunoprecipitation of MSLN transfected lysate using anti-MSLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MSLN MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MSLN is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MSLN.
Immunogen
MSLN (NP_005814, 464 a.a. ~ 563 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (58); Rat (57)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MSLN transfected lysate using anti-MSLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MSLN MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MSLN is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — MSLN
Entrez GeneID
10232GeneBank Accession#
NM_005823Protein Accession#
NP_005814Gene Name
MSLN
Gene Alias
CAK1, MPF, SMR
Gene Description
mesothelin
Omim ID
601051Gene Ontology
HyperlinkGene Summary
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
megakaryocyte potentiating factor
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Interactome
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Disease
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