PLIN3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PLIN3 transfected lysate.
Western Blot
Lane 1: PLIN3 transfected lysate ( 47 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PLIN3 full-length
Host
Human
Theoretical MW (kDa)
47
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PLIN3 antibody (H00010226-B01P) by Western Blots.
SDS-PAGE Gel
PLIN3 transfected lysate.
Western Blot
Lane 1: PLIN3 transfected lysate ( 47 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PLIN3
Entrez GeneID
10226GeneBank Accession#
NM_005817.3Protein Accession#
NP_005808.2Gene Name
PLIN3
Gene Alias
MGC11117, MGC2012, PP17, TIP47, M6PRBP1
Gene Description
mannose-6-phosphate receptor binding protein 1
Omim ID
602702Gene Ontology
HyperlinkGene Summary
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
MPR-BINDING PROTEIN, 47-KD|MPR-binding protein 47 kDa|cargo selection protein (mannose 6 phosphate receptor binding protein)|cargo selection protein TIP47|mannose 6 phosphate receptor binding protein 1|placental protein 17|tail-interacting protein, 47 kD
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