PLIN3 monoclonal antibody (M01), clone 2F7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PLIN3 expression in transfected 293T cell line by PLIN3 monoclonal antibody (M01), clone 2F7.
Lane 1: PLIN3 transfected lysate (Predicted MW: 47 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PLIN3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of PLIN3 transfected lysate using anti-PLIN3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLIN3 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLIN3 is 3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (73.48 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PLIN3.
Immunogen
PLIN3 (AAH01590, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHVKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQALSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (73.48 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PLIN3 expression in transfected 293T cell line by PLIN3 monoclonal antibody (M01), clone 2F7.
Lane 1: PLIN3 transfected lysate (Predicted MW: 47 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PLIN3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of PLIN3 transfected lysate using anti-PLIN3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLIN3 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLIN3 is 3 ng/ml as a capture antibody.ELISA
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Gene Info — PLIN3
Entrez GeneID
10226GeneBank Accession#
BC001590Protein Accession#
AAH01590Gene Name
PLIN3
Gene Alias
MGC11117, MGC2012, PP17, TIP47, M6PRBP1
Gene Description
mannose-6-phosphate receptor binding protein 1
Omim ID
602702Gene Ontology
HyperlinkGene Summary
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
MPR-BINDING PROTEIN, 47-KD|MPR-binding protein 47 kDa|cargo selection protein (mannose 6 phosphate receptor binding protein)|cargo selection protein TIP47|mannose 6 phosphate receptor binding protein 1|placental protein 17|tail-interacting protein, 47 kD
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Interactome
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