MPZL2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Product Description
Transfected Cell Line
293T
Plasmid
pCMV-EVA1 full-length
Host
Human
Theoretical MW (kDa)
23.76
Interspecies Antigen Sequence
Mouse (81); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EVA1 antibody (H00010205-B01) by Western Blots.
SDS-PAGE Gel
MPZL2 transfected lysate.
Western Blot
Lane 1: MPZL2 transfected lysate ( 23.76 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MPZL2
Entrez GeneID
10205GeneBank Accession#
NM_005797.2Protein Accession#
NP_005788.1Gene Name
MPZL2
Gene Alias
EVA, EVA1
Gene Description
myelin protein zero-like 2
Omim ID
604873Gene Ontology
HyperlinkGene Summary
Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. This gene is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
epithelial V-like antigen 1
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