TNK2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TNK2 transfected lysate.
Western Blot
Lane 1: TNK2 transfected lysate ( 39.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TNK2 full-length
Host
Human
Theoretical MW (kDa)
39.8
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TNK2 antibody (H00010188-D01P) by Western Blots.
SDS-PAGE Gel
TNK2 transfected lysate.
Western Blot
Lane 1: TNK2 transfected lysate ( 39.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TNK2
Entrez GeneID
10188GeneBank Accession#
BC008884.2Protein Accession#
AAH08884.1Gene Name
TNK2
Gene Alias
ACK, ACK1, FLJ44758, FLJ45547, p21cdc42Hs
Gene Description
tyrosine kinase, non-receptor, 2
Omim ID
606994Gene Ontology
HyperlinkGene Summary
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq
Other Designations
activated Cdc42-associated kinase 1|activated p21cdc42Hs kinase
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