YAF2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
YAF2 transfected lysate.
Western Blot
Lane 1: YAF2 transfected lysate ( 19.9 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-YAF2 full-length
Host
Human
Theoretical MW (kDa)
19.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-YAF2 antibody (H00010138-B01) by Western Blots.
SDS-PAGE Gel
YAF2 transfected lysate.
Western Blot
Lane 1: YAF2 transfected lysate ( 19.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — YAF2
Entrez GeneID
10138GeneBank Accession#
NM_005748.3Protein Accession#
-Gene Name
YAF2
Gene Alias
MGC41856
Gene Description
YY1 associated factor 2
Omim ID
607534Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. [provided by RefSeq
Other Designations
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Interactome
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Disease
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