ELA3A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ELA3A transfected lysate.
Western Blot
Lane 1: ELA3A transfected lysate ( 29.81 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ELA3A full-length
Host
Human
Theoretical MW (kDa)
29.81
Interspecies Antigen Sequence
Mouse (72)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ELA3A antibody (H00010136-B01) by Western Blots.
SDS-PAGE Gel
ELA3A transfected lysate.
Western Blot
Lane 1: ELA3A transfected lysate ( 29.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ELA3A
Entrez GeneID
10136GeneBank Accession#
NM_005747.3Protein Accession#
NP_005738.3Gene Name
ELA3A
Gene Alias
ELA3
Gene Description
elastase 3A, pancreatic
Gene Ontology
HyperlinkGene Summary
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq
Other Designations
OTTHUMP00000002835|elastase 1|protease E
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