TRAP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TRAP1 transfected lysate.
Western Blot
Lane 1: TRAP1 transfected lysate ( 77.55 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRAP1 full-length
Host
Human
Theoretical MW (kDa)
77.55
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRAP1 antibody (H00010131-B01) by Western Blots.
SDS-PAGE Gel
TRAP1 transfected lysate.
Western Blot
Lane 1: TRAP1 transfected lysate ( 77.55 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRAP1
Entrez GeneID
10131GeneBank Accession#
NM_016292.1Protein Accession#
NP_057376.1Gene Name
TRAP1
Gene Alias
HSP75, HSP90L
Gene Description
TNF receptor-associated protein 1
Omim ID
606219Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock protein 75|tumor necrosis factor type 1 receptor associated protein
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Interactome
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Disease
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