ACTR1A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ACTR1A transfected lysate.
Western Blot
Lane 1: ACTR1A transfected lysate ( 42.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACTR1A full-length
Host
Human
Theoretical MW (kDa)
42.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACTR1A antibody (H00010121-B01) by Western Blots.
SDS-PAGE Gel
ACTR1A transfected lysate.
Western Blot
Lane 1: ACTR1A transfected lysate ( 42.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACTR1A
Entrez GeneID
10121GeneBank Accession#
NM_005736.2Protein Accession#
-Gene Name
ACTR1A
Gene Alias
ARP1, CTRN1, FLJ52695, FLJ52800, FLJ55002
Gene Description
ARP1 actin-related protein 1 homolog A, centractin alpha (yeast)
Omim ID
605143Gene Ontology
HyperlinkGene Summary
This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin. [provided by RefSeq
Other Designations
ARP1 actin-related protein 1 homolog A, centractin alpha|ARP1, yeast homolog A|OTTHUMP00000020366|actin-RPV|centractin alpha|centrosome-associated actin homolog
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