TSFM 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
TSFM transfected lysate.
Western Blot
Lane 1: TSFM transfected lysate ( 38.17 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TSFM full-length
Host
Human
Theoretical MW (kDa)
38.17
Interspecies Antigen Sequence
Mouse (80); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TSFM antibody (H00010102-B01) by Western Blots.
SDS-PAGE Gel
TSFM transfected lysate.
Western Blot
Lane 1: TSFM transfected lysate ( 38.17 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TSFM
Entrez GeneID
10102GeneBank Accession#
NM_005726.2Protein Accession#
-Gene Name
TSFM
Gene Alias
COXPD3, EF-TS, EF-Tsmt
Gene Description
Ts translation elongation factor, mitochondrial
Gene Ontology
HyperlinkGene Summary
Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM
Other Designations
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