PQBP1 monoclonal antibody (M01), clone 1A11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PQBP1 expression in transfected 293T cell line by PQBP1 monoclonal antibody (M01), clone 1A11.
Lane 1: PQBP1 transfected lysate(30.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PQBP1 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (34.76 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PQBP1.
Immunogen
PQBP1 (NP_005701, 184 a.a. ~ 265 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LAPYPKSKKAVSRKDEELDPMDPSSYSDAPRGTWSTGLPKRNEAKTGADTTAAGPLFQQRPYPSPGAVLRANAEASRTKQQD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.76 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PQBP1 expression in transfected 293T cell line by PQBP1 monoclonal antibody (M01), clone 1A11.
Lane 1: PQBP1 transfected lysate(30.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PQBP1 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — PQBP1
Entrez GeneID
10084GeneBank Accession#
NM_005710Protein Accession#
NP_005701Gene Name
PQBP1
Gene Alias
MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS
Gene Description
polyglutamine binding protein 1
Gene Ontology
HyperlinkGene Summary
PQBP1 is a nuclear polyglutamine-binding protein that contains a WW domain (Waragai et al., 1999 [PubMed 10332029]).[supplied by OMIM
Other Designations
OTTHUMP00000025808|nuclear protein containing WW domain 38 kD
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Interactome
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Publication Reference
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RNA and protein interactors with TDP-43 in human spinal cord lysates in ALS.
Volkening K, Keller B, Leysta-Lantz C, Strong MJ.
Journal of Proteome Research 2018 Apr; 17(4):1712.
Application：WB, Human, HEK293T cells, lumbar spinal cord lysates.
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RNA and protein interactors with TDP-43 in human spinal cord lysates in ALS.
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