DNAJB6 monoclonal antibody (M02), clone 2D12-B9
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More Files
- More Functions
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant DNAJB6.
Immunogen
DNAJB6 (AAH00177, 1 a.a. ~ 241 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (75); Rat (78)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody (M02), clone 2D12-B9.
Lane 1: DNAJB6 transfected lysate (Predicted MW: 36.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of DNAJB6 transfected lysate using anti-DNAJB6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DNAJB6 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DNAJB6 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DNAJB6 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — DNAJB6
Entrez GeneID
10049GeneBank Accession#
BC000177Protein Accession#
AAH00177Gene Name
DNAJB6
Gene Alias
DJ4, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-2, HSJ2, MGC1152, MGC117297, MRJ, MSJ-1
Gene Description
DnaJ (Hsp40) homolog, subfamily B, member 6
Omim ID
611332Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq
Other Designations
DnaJ-like 2 protein|heat shock protein J2
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Interactome
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