DNAJB6 monoclonal antibody (M01), clone 2C11-C1
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant DNAJB6.
Immunogen
DNAJB6 (AAH00177, 1 a.a. ~ 241 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (75); Rat (78)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DNAJB6 monoclonal antibody (M01), clone 2C11-C1. Western Blot analysis of DNAJB6 expression in C32 ( Cat # L002V1 ).Western Blot (Cell lysate)
DNAJB6 monoclonal antibody (M01), clone 2C11-C1 Western Blot analysis of DNAJB6 expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody (M01), clone 2C11-C1.
Lane 1: DNAJB6 transfected lysate(27 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DNAJB6 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — DNAJB6
Entrez GeneID
10049GeneBank Accession#
BC000177Protein Accession#
AAH00177Gene Name
DNAJB6
Gene Alias
DJ4, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-2, HSJ2, MGC1152, MGC117297, MRJ, MSJ-1
Gene Description
DnaJ (Hsp40) homolog, subfamily B, member 6
Omim ID
611332Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq
Other Designations
DnaJ-like 2 protein|heat shock protein J2
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Interactome
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Publication Reference
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The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss.
Hong YM, Hong Y, Choi YG, Yeo S, Jin SH, Lee SW, Sung B, Lee SH, Jung H, Lim S.
Amino Acids 2017 Feb; 2017:7982389.
Application:IF, WB-Tr, Human, LN18 cells.
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Interaction between urokinase receptor and heat shock protein MRJ enhances cell adhesion.
Edo De Bock C, Lin Z, Mekkawy AH, Byrne JA, Wang Y.
International Journal of Oncology 2010 May; 36(5):1155.
Application:WB-Ce, Human, MDA-MB-231 cells.
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The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss.
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