ACOT8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACOT8 transfected lysate.
Western Blot
Lane 1: ACOT8 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACOT8 full-length
Host
Human
Theoretical MW (kDa)
35.2
Interspecies Antigen Sequence
Mouse (85); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACOT8 antibody (H00010005-B01) by Western Blots.
SDS-PAGE Gel
ACOT8 transfected lysate.
Western Blot
Lane 1: ACOT8 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACOT8
Entrez GeneID
10005GeneBank Accession#
NM_005469.2Protein Accession#
NP_005460.2Gene Name
ACOT8
Gene Alias
HNAACTE, PTE-2, PTE1, PTE2, hACTE-III, hTE
Gene Description
acyl-CoA thioesterase 8
Omim ID
608123Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HIV-Nef associated acyl-CoA thioesterase|OTTHUMP00000031675|long-chain fatty-acyl-CoA hydrolase|palmitoyl-CoA hydrolase|peroxisomal acyl-CoA thioesterase 1|peroxisomal acyl-coenzyme A thioester hydrolase 1|peroxisomal long-chain acyl-CoA thioesterase 1|th
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