NR1D2 monoclonal antibody (M01), clone 1G2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NR1D2.
Immunogen
NR1D2 (AAH45613, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEVNAGGVIAYISSSSSASSHASCHSEGSENSFQSSSSSVPSSPNSSNSDTNGNPKNGDLANIEGILKNDRIDCSMKTSKSSAPGMTKSHSGVTKFSGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKKCLKNENCSIMRMNRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLIEMQSAMKTMMNSQFSGHLQNDTLVEHHEQTALPAQEQLRPKPQLEQENIKSSSPPSSDFAKEEVIGMVTRAHKDTFMYNQEQQENSAESMQPKRGERIRKNMEQYNLNHDHCGNGLSSHFPCSESQQHLNGQFKGRNIMHYPNGHAICIANGHCMNFSNAYTQRVCDRVPIDGFSQNENKNSYLCNTGGRMHLVCPMSKSPYVDPHKSGHEIWEEFSMSFTPAVKEVVEFAKRIPGFRDLSQHDQVNLLKAGTFEVLMVRFASLFDAKERTVTFLSGKKYSVDDLHSMGAGDLLNSMFEFSEKLNALQLSDEEMSLFTAVVLVSADRSGIENVNSVEALQETLIRALRTLIMKNHPNEASIFTKLLLKLPDLRSLNNMHSEELLAFKVHP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (89.21 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NR1D2 monoclonal antibody (M01), clone 1G2 Western Blot analysis of NR1D2 expression in Hela ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NR1D2 expression in transfected 293T cell line by NR1D2 monoclonal antibody (M01), clone 1G2.
Lane 1: NR1D2 transfected lysate(64.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of NR1D2 over-expressed 293 cell line, cotransfected with NR1D2 Validated Chimera RNAi ( Cat # H00009975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D2 monoclonal antibody (M01), clone 1G2 (Cat # H00009975-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to NR1D2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NR1D2
Entrez GeneID
9975GeneBank Accession#
BC045613Protein Accession#
AAH45613Gene Name
NR1D2
Gene Alias
BD73, EAR-1r, HZF2, Hs.37288, RVR
Gene Description
nuclear receptor subfamily 1, group D, member 2
Omim ID
602304Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
Rev-erb-beta
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Interactome
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