GOLGA5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
GOLGA5 transfected lysate.
Western Blot
Lane 1: GOLGA5 transfected lysate ( 80.52 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GOLGA5 full-length
Host
Human
Theoretical MW (kDa)
80.52
Interspecies Antigen Sequence
Mouse (83); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GOLGA5 antibody (H00009950-B01) by Western Blots.
SDS-PAGE Gel
GOLGA5 transfected lysate.
Western Blot
Lane 1: GOLGA5 transfected lysate ( 80.52 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GOLGA5
Entrez GeneID
9950GeneBank Accession#
NM_005113.2Protein Accession#
NP_005104.2Gene Name
GOLGA5
Gene Alias
GOLIM5, RFG5, ret-II
Gene Description
golgi autoantigen, golgin subfamily a, 5
Gene Ontology
HyperlinkGene Summary
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq
Other Designations
Golgi autoantigen, golgin subfamily a, 5|cell proliferation-inducing gene 31|golgi integral membrane protein 5|golgin-84|ret-fused gene 5
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