RBM8A monoclonal antibody (M08), clone 3E4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of RBM8A expression in transfected 293T cell line by RBM8A monoclonal antibody (M08), clone 3E4.
Lane 1: RBM8A transfected lysate (Predicted MW: 19.9 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RBM8A on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RBM8A is 3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (44.88 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant RBM8A.
Immunogen
RBM8A (AAH17088, 1 a.a. ~ 174 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MADVLDLHEAGGEDFAMDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQDGDEPGPQRSVEGWILSVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNGQDLMGQPISVDWCFVRGPPKGKRRGGRRRSRSPDRRRR
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (44.88 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RBM8A expression in transfected 293T cell line by RBM8A monoclonal antibody (M08), clone 3E4.
Lane 1: RBM8A transfected lysate (Predicted MW: 19.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RBM8A on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RBM8A is 3 ng/ml as a capture antibody.ELISA
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Gene Info — RBM8A
Entrez GeneID
9939GeneBank Accession#
BC017088Protein Accession#
AAH17088Gene Name
RBM8A
Gene Alias
BOV-1A, BOV-1B, BOV-1C, MDS014, RBM8, RBM8B, Y14, ZNRP, ZRNP1
Gene Description
RNA binding motif protein 8A
Omim ID
605313Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites. [provided by RefSeq
Other Designations
OTTHUMP00000015573|RNA binding motif protein 8B|binder of OVCA1-1|ribonucleoprotein RBM8
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