GINS1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GINS1 full-length
Host
Human
Theoretical MW (kDa)
21.67
Interspecies Antigen Sequence
Mouse (92); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GINS1 antibody (H00009837-B02) by Western Blots.
SDS-PAGE Gel
GINS1 transfected lysate.
Western Blot
Lane 1: GINS1 transfected lysate ( 21.67 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GINS1
Entrez GeneID
9837GeneBank Accession#
BC012542Protein Accession#
AAH12542Gene Name
GINS1
Gene Alias
KIAA0186, PSF1
Gene Description
GINS complex subunit 1 (Psf1 homolog)
Omim ID
610608Gene Ontology
HyperlinkGene Summary
The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611), Psf1, Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]).[supplied by OMIM
Other Designations
DNA replication complex GINS protein PSF1|OTTHUMP00000030496|partner of sld five-1
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