This antibody pair set comes with matched antibody pair to detect and quantify protein level of human GIT2.
Quality Control Testing:
Standard curve using GIT2 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 27x to 3x dilution of the GIT2 293T overexpression lysate (non-denatured).
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-GIT2 (100 ug) 2. Detection antibody: rabbit purified polyclonal anti-GIT2 (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq
ARF GTPase-activating protein GIT2,G protein-coupled receptor kinase interactor 2,GRK-interacting protein 2,cool-associated, tyrosine phosphorylated protein 2