DAZAP2 monoclonal antibody (M06), clone 8G5
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More Files
- More Functions
Western Blot (Cell lysate)
DAZAP2 monoclonal antibody (M06), clone 8G5. Western Blot analysis of DAZAP2 expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of DAZAP2 expression in transfected 293T cell line by DAZAP2 monoclonal antibody (M06), clone 8G5.
Lane 1: DAZAP2 transfected lysate (Predicted MW: 17.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DAZAP2 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (43.7 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant DAZAP2.
Immunogen
DAZAP2 (NP_055579.1, 1 a.a. ~ 168 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNSKGQYPTQPTYPVQPPGNPVYPQTLHLPQAPPYTDAPPAYSELYRPSFVHPGAATVPTMSAAFPGASLYLPMAQSVAVGPLGSTIPMAYYPVGPIYPPGSTVLVEGGYDAGARFGAGATAGNIPPPPPGCPPNAAQLAVMQGANVLVTQRKGNFFMGGSDGGYTIW
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (43.7 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DAZAP2 monoclonal antibody (M06), clone 8G5. Western Blot analysis of DAZAP2 expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of DAZAP2 expression in transfected 293T cell line by DAZAP2 monoclonal antibody (M06), clone 8G5.
Lane 1: DAZAP2 transfected lysate (Predicted MW: 17.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DAZAP2 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — DAZAP2
Entrez GeneID
9802GeneBank Accession#
NM_014764.2Protein Accession#
NP_055579.1Gene Name
DAZAP2
Gene Alias
KIAA0058, MGC14319, MGC766, PRTB
Gene Description
DAZ associated protein 2
Omim ID
607431Gene Ontology
HyperlinkGene Summary
This gene encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
deleted in azoospermia associated protein 2|proline-rich transcript in brain|proline-rich transcript, brain-expressed protein
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