RAPGEF5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
RAPGEF5 transfected lysate.
Western Blot
Lane 1: RAPGEF5 transfected lysate ( 48.95 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RAPGEF5 full-length
Host
Human
Theoretical MW (kDa)
48.95
Interspecies Antigen Sequence
Mouse (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RAPGEF5 antibody (H00009771-B01P) by Western Blots.
SDS-PAGE Gel
RAPGEF5 transfected lysate.
Western Blot
Lane 1: RAPGEF5 transfected lysate ( 48.95 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RAPGEF5
Entrez GeneID
9771GeneBank Accession#
BC039203Protein Accession#
AAH39203Gene Name
RAPGEF5
Gene Alias
GFR, KIAA0277, MR-GEF, REPAC
Gene Description
Rap guanine nucleotide exchange factor (GEF) 5
Omim ID
609527Gene Ontology
HyperlinkGene Summary
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM
Other Designations
M-Ras-regulated GEF|guanine nucleotide exchange factor for Rap1
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Interactome
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Disease
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