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RAPGEF5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00009771-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RAPGEF5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 48.95
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RAPGEF5 antibody (H00009771-B01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00009771-T02
    RAPGEF5 transfected lysate.
    Western Blot
    QC Testing of H00009771-T02
    Lane 1: RAPGEF5 transfected lysate ( 48.95 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 9771
  • Gene Name:
  • RAPGEF5
  • Gene Alias:
  • GFR,KIAA0277,MR-GEF,REPAC
  • Gene Description:
  • Rap guanine nucleotide exchange factor (GEF) 5
  • Gene Summary:
  • Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM
  • Other Designations:
  • M-Ras-regulated GEF,guanine nucleotide exchange factor for Rap1
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