PUM1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PUM1 transfected lysate.
Western Blot
Lane 1: PUM1 transfected lysate ( 126.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PUM1 full-length
Host
Human
Theoretical MW (kDa)
126.5
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PUM1 antibody (H00009698-B01) by Western Blots.
SDS-PAGE Gel
PUM1 transfected lysate.
Western Blot
Lane 1: PUM1 transfected lysate ( 126.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PUM1
Entrez GeneID
9698GeneBank Accession#
NM_014676Protein Accession#
NP_055491Gene Name
PUM1
Gene Alias
HSPUM, KIAA0099, PUMH, PUMH1, PUML1
Gene Description
pumilio homolog 1 (Drosophila)
Omim ID
607204Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000003892|pumilio 1
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