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Last updated: 2017/4/23

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EIF5B 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00009669-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-EIF5B full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 134.31
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-EIF5B antibody (H00009669-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00009669-T01
    EIF5B transfected lysate.
    Western Blot
    QC Testing of H00009669-T01
    Lane 1: EIF5B transfected lysate ( 134.31 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 9669
  • Gene Name:
  • EIF5B
  • Gene Alias:
  • DKFZp434I036,FLJ10524,IF2,KIAA0741
  • Gene Description:
  • eukaryotic translation initiation factor 5B
  • Gene Summary:
  • Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq
  • Other Designations:
  • translation initiation factor IF2
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