EIF5B purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
EIF5B MaxPab polyclonal antibody. Western Blot analysis of EIF5B expression in human placenta.
Western Blot (Cell lysate)
EIF5B MaxPab polyclonal antibody. Western Blot analysis of EIF5B expression in Jurkat.
Western Blot (Transfected lysate)
Western Blot analysis of EIF5B expression in transfected 293T cell line (H00009669-T01) by EIF5B MaxPab polyclonal antibody.
Lane 1: EIF5B transfected lysate(134.2 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to EIF5B on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human EIF5B protein.
Immunogen
EIF5B (AAH32639.1, 1 a.a. ~ 1220 a.a) full-length human protein.
Sequence
MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNTVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVNLAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (89)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
EIF5B MaxPab polyclonal antibody. Western Blot analysis of EIF5B expression in human placenta.Western Blot (Cell lysate)
EIF5B MaxPab polyclonal antibody. Western Blot analysis of EIF5B expression in Jurkat.Western Blot (Transfected lysate)
Western Blot analysis of EIF5B expression in transfected 293T cell line (H00009669-T01) by EIF5B MaxPab polyclonal antibody.
Lane 1: EIF5B transfected lysate(134.2 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to EIF5B on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — EIF5B
Entrez GeneID
9669GeneBank Accession#
BC032639.1Protein Accession#
AAH32639.1Gene Name
EIF5B
Gene Alias
DKFZp434I036, FLJ10524, IF2, KIAA0741
Gene Description
eukaryotic translation initiation factor 5B
Omim ID
606086Gene Ontology
HyperlinkGene Summary
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq
Other Designations
translation initiation factor IF2
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Interactome
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Publication Reference
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Association of EV71 3C polymorphisms with clinical severity.
Ma HY, Lu CY, Tsao KC, Shih HM, Cheng AL, Huang LM, Chang LY.
Journal of Microbiology, Immunology, and Infection 2017 Jul; [Epub].
Application：WB-Tr, Human, SF268 cells.
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Association of EV71 3C polymorphisms with clinical severity.
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