ISG15 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ISG15 full-length
Host
Human
Theoretical MW (kDa)
17.9
Interspecies Antigen Sequence
Mouse (65); Rat (63)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ISG15 antibody (H00009636-B01) by Western Blots.
SDS-PAGE Gel
G1P2 transfected lysate.
Western Blot
Lane 1: G1P2 transfected lysate ( 17.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ISG15
Entrez GeneID
9636GeneBank Accession#
NM_005101.1Protein Accession#
-Gene Name
ISG15
Gene Alias
G1P2, IFI15, UCRP
Gene Description
ISG15 ubiquitin-like modifier
Omim ID
147571Gene Ontology
HyperlinkGene Summary
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM
Other Designations
OTTHUMP00000038464|interferon, alpha-inducible protein (clone IFI-15K)|interferon-induced 17-kDa/15-kDa protein|interferon-stimulated protein, 15 kDa|ubiquitin cross-reactive protein
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Interactome
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Disease
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