TRAF4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TRAF4 transfected lysate.
Western Blot
Lane 1: TRAF4 transfected lysate ( 53.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRAF4 full-length
Host
Human
Theoretical MW (kDa)
53.5
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRAF4 antibody (H00009618-D01P) by Western Blots.
SDS-PAGE Gel
TRAF4 transfected lysate.
Western Blot
Lane 1: TRAF4 transfected lysate ( 53.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRAF4
Entrez GeneID
9618GeneBank Accession#
NM_004295Protein Accession#
NP_004286.2Gene Name
TRAF4
Gene Alias
CART1, MLN62, RNF83
Gene Description
TNF receptor-associated factor 4
Omim ID
602464Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq
Other Designations
cysteine-rich domain associated with ring and TRAF domain|malignant 62|tumor necrosis receptor-associated factor 4A
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Interactome
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Pathway
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Disease
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