RNF14 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNF14 transfected lysate.
Western Blot
Lane 1: RNF14 transfected lysate ( 39.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF14 full-length
Host
Human
Theoretical MW (kDa)
39.6
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF14 antibody (H00009604-D01P) by Western Blots.
SDS-PAGE Gel
RNF14 transfected lysate.
Western Blot
Lane 1: RNF14 transfected lysate ( 39.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF14
Entrez GeneID
9604GeneBank Accession#
NM_183398.1Protein Accession#
NP_899645.1Gene Name
RNF14
Gene Alias
ARA54, FLJ26004, HFB30, HRIHFB2038, TRIAD2
Gene Description
ring finger protein 14
Omim ID
605675Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq
Other Designations
androgen receptor associated protein 54|triad2 protein
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