Products

Western Blot (Transfected lysate)

Western Blot analysis of RNF14 expression in transfected 293T cell line by RNF14 monoclonal antibody (M01), clone 4G9.

Lane 1: RNF14 transfected lysate(39.6 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged RNF14 is approximately 0.1ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14 Validated Chimera RNAi ( Cat # H00009604-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF14 monoclonal antibody (M01), clone 4G9 (Cat # H00009604-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to RNF14 on HeLa cell . [antibody concentration 10 ug/ml]

Entrez GeneID

9604

GeneBank Accession#

NM_004290

Protein Accession#

NP_004281

Gene Name

RNF14

Gene Alias

ARA54, FLJ26004, HFB30, HRIHFB2038, TRIAD2

Gene Description

ring finger protein 14

Omim ID

605675

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq

Other Designations

androgen receptor associated protein 54|triad2 protein