AKAP12 monoclonal antibody (M01), clone 1C5
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.62 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant AKAP12.
Immunogen
AKAP12 (NP_005091, 1675 a.a. ~ 1782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (56); Rat (56)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.62 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.ELISA
-
Gene Info — AKAP12
Entrez GeneID
9590GeneBank Accession#
NM_005100Protein Accession#
NP_005091Gene Name
AKAP12
Gene Alias
AKAP250, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621
Gene Description
A kinase (PRKA) anchor protein 12
Omim ID
604698Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
A kinase (PRKA) anchor protein (gravin) 12|A-kinase anchor protein, 250kDa|OTTHUMP00000017426|kinase scaffold protein gravin|myasthenia gravis autoantigen gravin
-
Interactome
-
Disease
-
Publication Reference
-
iTRAQ analysis of colorectal cancer cell lines suggests Drebrin (DBN1) is overexpressed during liver metastasis.
Lin Q, Tan HT, Lim TK, Khoo A, Lim KH, Chung MC.
Proteomics 2014 Jun; 14(11):1434.
Application：WB-Ce, Human, HCT-116, E1 cells.
-
Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells.
Tan HT, Tan S, Lin Q, Lim TK, Hew CL, Chung MC.
Molecular & Cellular Proteomics 2008 Mar; 7(6):1174.
Application：WB, Human, HCT-116.
-
iTRAQ analysis of colorectal cancer cell lines suggests Drebrin (DBN1) is overexpressed during liver metastasis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com