WTAP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WTAP full-length
Host
Human
Theoretical MW (kDa)
43.67
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WTAP antibody (H00009589-B01) by Western Blots.
SDS-PAGE Gel
WTAP transfected lysate.
Western Blot
Lane 1: WTAP transfected lysate ( 43.67 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WTAP
Entrez GeneID
9589GeneBank Accession#
NM_004906.3Protein Accession#
NP_004897.2Gene Name
WTAP
Gene Alias
DKFZp686F20131, KIAA0105, MGC3925
Gene Description
Wilms tumor 1 associated protein
Omim ID
605442Gene Ontology
HyperlinkGene Summary
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq
Other Designations
OTTHUMP00000017522|OTTHUMP00000017523|PNAS-132|WT1-associated protein|Wilms' tumour 1-associating protein|putative pre-mRNA splicing regulator female-lethal(2D)
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