MAD2L1BP monoclonal antibody (M03), clone 4G11
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MAD2L1BP.
Immunogen
MAD2L1BP (AAH02904, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAPEAEVLSSAAVPDLEWYEKSEETHASQIELLETSSTQEPLNASEAFCPRDCMVPVVFPGPVSQEGCCQFTCELLKHIMYQRQQLPLPYEQLKHFYRKPSPQAEEMLKKKPRATTEVSSRKCQQALAELESVLSHLEDFFARTLVPRVLILLGGNALSPKEFYELDLSLLAPYSVDQSLSTAACLRRLFRAIFMADAFSELQAPPLMGTVVMAQGHRNCGEDWFRPKLNYRVPSRGHKLTVTLSCGRPSIRTTAWEDYIWFQAPVTFKGFRE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (76); Rat (79)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.88 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MAD2L1BP monoclonal antibody (M03), clone 4G11. Western Blot analysis of MAD2L1BP expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAD2L1BP is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAD2L1BP on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MAD2L1BP
Entrez GeneID
9587GeneBank Accession#
BC002904Protein Accession#
AAH02904Gene Name
MAD2L1BP
Gene Alias
CMT2, KIAA0110, MGC11282, RP1-261G23.6
Gene Description
MAD2L1 binding protein
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000016496|caught by MAD2 protein
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