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Last updated: 2016/10/23
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SPAG6 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00009576-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SPAG6 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 55.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SPAG6 antibody (H00009576-B01) by Western Blots.
    Western Blot
    QC Testing of H00009576-T01
    Lane 1: SPAG6 transfected lysate ( 55.5 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00009576-T01
    SPAG6 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 9576
  • Protein Accession#:
  • -
  • Gene Name:
  • SPAG6
  • Gene Alias:
  • DKFZp434I153,MGC26276,Repro-SA-1,pf16
  • Gene Description:
  • sperm associated antigen 6
  • Gene Summary:
  • The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019296,OTTHUMP00000019297,axoneme central apparatus protein,sperm flagellar protein
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