NR1D1 monoclonal antibody (M02), clone 4F6
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More Files
- More Functions
Western Blot (Cell lysate)
NR1D1 monoclonal antibody (M02), clone 4F6. Western Blot analysis of NR1D1 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot (Cell lysate)
NR1D1 monoclonal antibody (M02), clone 4F6 Western Blot analysis of NR1D1 expression in Hela S3 NE ( Cat # L013V3 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to NR1D1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (93.28 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NR1D1.
Immunogen
NR1D1 (AAH56148, 1 a.a. ~ 614 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (93.28 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NR1D1 monoclonal antibody (M02), clone 4F6. Western Blot analysis of NR1D1 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
NR1D1 monoclonal antibody (M02), clone 4F6 Western Blot analysis of NR1D1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to NR1D1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NR1D1
Entrez GeneID
9572GeneBank Accession#
BC056148Protein Accession#
AAH56148Gene Name
NR1D1
Gene Alias
EAR1, THRA1, THRAL, ear-1, hRev
Gene Description
nuclear receptor subfamily 1, group D, member 1
Omim ID
602408Gene Ontology
HyperlinkGene Summary
group D
Other Designations
Rev-ErbAalpha|thyroid hormone receptor, alpha-like
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Interactome
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Pathway
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Disease
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Publication Reference
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NR1D1 downregulation in astrocytes induces a phenotype that is detrimental to cocultured motor neurons.
Kelby M Killoy, Benjamin A Harlan, Mariana Pehar, Marcelo R Vargas.
FASEB Journal 2022 Apr; 36(4):e22262.
Application：IF, Mouse, Lumbar spinal cord sections.
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Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M.
PLoS One 2017 Dec; 12(12):e0188772.
Application：IF, Human, HeLa cells.
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Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.
DeBruyne JP, Baggs JE, Sato TK, Hogenesch JB.
Proceedings of the National Academy of Sciences of the United States of America 2015 Oct; 112(40):12420.
Application：WB, Human, U2OS cells.
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Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1β gene expression in pancreatic islet β-cells.
Nakabayashi H, Ohta Y, Yamamoto M, Susuki Y, Taguchi A, Tanabe K, Kondo M, Hatanaka M, Nagao Y, Tanizawa Y.
Biochemical and Biophysical Research Communications 2013 May; 434(2):370.
Application：WB-Tr, ChIP, Human, Mouse, HEK 293 cells, MIN6 cells.
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NR1D1 downregulation in astrocytes induces a phenotype that is detrimental to cocultured motor neurons.
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