BAG2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BAG2 full-length
Host
Human
Theoretical MW (kDa)
23.32
Interspecies Antigen Sequence
Mouse (93); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BAG2 antibody (H00009532-B01) by Western Blots.
Western Blot
Lane 1: BAG2 transfected lysate ( 23.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
BAG2 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BAG2
Entrez GeneID
9532GeneBank Accession#
NM_004282Protein Accession#
NP_004273Gene Name
BAG2
Gene Alias
BAG-2, KIAA0576, MGC149462, dJ417I1.2
Gene Description
BCL2-associated athanogene 2
Omim ID
603882Gene Ontology
HyperlinkGene Summary
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq
Other Designations
BAG-family molecular chaperone regulator-2|OTTHUMP00000016668|dJ417I1.2 (BAG-family molecular chaperone regulator 2)
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