BAG3 monoclonal antibody (M01A), clone 5A8
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of BAG3 expression in transfected 293T cell line by BAG3 monoclonal antibody (M01A), clone 5A8.
Lane 1: BAG3 transfected lysate (Predicted MW: 61.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (88.99 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant BAG3.
Immunogen
BAG3 (AAH14656.1, 1 a.a. ~ 575 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSAATHSPMMQVASGNGDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQCGQVAAAAAAQPPASHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (85)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (88.99 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of BAG3 expression in transfected 293T cell line by BAG3 monoclonal antibody (M01A), clone 5A8.
Lane 1: BAG3 transfected lysate (Predicted MW: 61.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — BAG3
Entrez GeneID
9531GeneBank Accession#
BC014656Protein Accession#
AAH14656.1Gene Name
BAG3
Gene Alias
BAG-3, BIS, CAIR-1, MGC104307
Gene Description
BCL2-associated athanogene 3
Omim ID
603883Gene Ontology
HyperlinkGene Summary
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq
Other Designations
BAG-family molecular chaperone regulator-3|BCL2-binding athanogene 3|Bcl-2-binding protein|OTTHUMP00000020599|docking protein CAIR-1
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Interactome
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Disease
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Publication Reference
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Co-chaperone BAG3 enters autophagic pathway via its interaction with microtubule associated protein 1 light chain 3 beta.
Hagen Körschgen, Marius Baeken, Daniel Schmitt, Heike Nagel, Christian Behl.
Traffic (Copenhagen, Denmark) 2023 Sep; [Epub].
Application：PLA, WB, Human, HeLa cells.
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Co-chaperone BAG3 enters autophagic pathway via its interaction with microtubule associated protein 1 light chain 3 beta.
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