EEF1E1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
EEF1E1 transfected lysate.
Western Blot
Lane 1: EEF1E1 transfected lysate ( 19.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EEF1E1 full-length
Host
Human
Theoretical MW (kDa)
19.8
Interspecies Antigen Sequence
Mouse (88); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EEF1E1 antibody (H00009521-B01) by Western Blots.
SDS-PAGE Gel
EEF1E1 transfected lysate.
Western Blot
Lane 1: EEF1E1 transfected lysate ( 19.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EEF1E1
Entrez GeneID
9521GeneBank Accession#
NM_004280.2Protein Accession#
-Gene Name
EEF1E1
Gene Alias
AIMP3, P18
Gene Description
eukaryotic translation elongation factor 1 epsilon 1
Omim ID
609206Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
ARS-interacting multifunctional protein 3|OTTHUMP00000016008|p18 component of aminoacyl-tRNA synthetase complex
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