SLC25A27 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SLC25A27 transfected lysate
Western Blot
Lane 1: SLC25A27 transfected lysate ( 27.06 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SLC25A27 full-length
Host
Human
Theoretical MW (kDa)
27.06
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SLC25A27 antibody (H00009481-B01) by Western Blots.
SDS-PAGE Gel
SLC25A27 transfected lysate
Western Blot
Lane 1: SLC25A27 transfected lysate ( 27.06 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SLC25A27
Entrez GeneID
9481GeneBank Accession#
BC033091Protein Accession#
AAH33091Gene Name
SLC25A27
Gene Alias
FLJ33552, UCP4
Gene Description
solute carrier family 25, member 27
Gene Ontology
HyperlinkGene Summary
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. [provided by RefSeq
Other Designations
OTTHUMP00000016548|uncoupling protein 4
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Interactome
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Disease
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