MED20 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Cell lysate)
TRFP MaxPab polyclonal antibody. Western Blot analysis of TRFP expression in Hela S3 NE.
Western Blot (Transfected lysate)
Western Blot analysis of MED20 expression in transfected 293T cell line (H00009477-T01) by MED20 MaxPab polyclonal antibody.
Lane 1: TRFP transfected lysate(23.32 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to TRFP on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human MED20 protein.
Immunogen
MED20 (NP_004266.2, 1 a.a. ~ 212 a.a) full-length human protein.
Sequence
MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (98)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TRFP MaxPab polyclonal antibody. Western Blot analysis of TRFP expression in Hela S3 NE.Western Blot (Transfected lysate)
Western Blot analysis of MED20 expression in transfected 293T cell line (H00009477-T01) by MED20 MaxPab polyclonal antibody.
Lane 1: TRFP transfected lysate(23.32 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to TRFP on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MED20
Entrez GeneID
9477GeneBank Accession#
NM_004275.3Protein Accession#
NP_004266.2Gene Name
MED20
Gene Alias
DKFZp586D2223, MGC29869, PRO0213, TRFP
Gene Description
mediator complex subunit 20
Gene Ontology
HyperlinkGene Summary
MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM
Other Designations
OTTHUMP00000016389|OTTHUMP00000039764|Trf (TATA binding protein-related factor)-proximal homolog
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Interactome
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