ATG5 monoclonal antibody (M04), clone 1G11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ATG5.
Immunogen
ATG5 (AAH02699, 176 a.a. ~ 275 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ATG5 monoclonal antibody (M04), clone 1G11. Western Blot analysis of ATG5 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Cell lysate)
ATG5 monoclonal antibody (M04), clone 1G11. Western Blot analysis of ATG5 expression in Raw 264.7(Cat # L024V1 ).Western Blot (Cell lysate)
ATG5 monoclonal antibody (M04), clone 1G11. Western Blot analysis of ATG5 expression in Jurkat ( Cat # L017V1 ).Western Blot (Recombinant protein)
ELISA
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Gene Info — ATG5
Entrez GeneID
9474GeneBank Accession#
BC002699Protein Accession#
AAH02699Gene Name
ATG5
Gene Alias
APG5, APG5-LIKE, APG5L, ASP, hAPG5
Gene Description
ATG5 autophagy related 5 homolog (S. cerevisiae)
Omim ID
604261Gene Ontology
HyperlinkGene Summary
S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
Other Designations
APG5 (autophagy 5, S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Atg5flox-Derived Autophagy-Deficient Model of Pompe Disease: Does It Tell the Whole Story?
Lim JA, Zare H, Puertollano R, Raben N.
Molecular Therapy. Methods & Clinical development 2017 Sep; 7:11.
Application:WB-Ti, Mouse, Mouse muscles.
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Autophagy contributes to the survival of CD133+ liver cancer stem cells in the hypoxic and nutrient-deprived tumor microenvironment.
Song YJ, Zhang SS, Guo XL, Sun K, Han ZP, Li R, Zhao QD, Deng WJ, Xie XQ, Zhang JW, Wu MC, Wei LX.
Cancer Letters 2013 Oct; 339(1):70.
Application:WB, Human, Huh7 cells.
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Atg5flox-Derived Autophagy-Deficient Model of Pompe Disease: Does It Tell the Whole Story?
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