SH3BP5 monoclonal antibody (M01), clone 2B3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SH3BP5.
Immunogen
SH3BP5 (NP_004835, 4 a.a. ~ 112 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GLEEEEEVDPRIQGELEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (94)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SH3BP5 monoclonal antibody (M01), clone 2B3 Western Blot analysis of SH3BP5 expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SH3BP5 on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SH3BP5 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SH3BP5 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SH3BP5
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Interactome
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Publication Reference
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JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death.
Win S, Than TA, Fernandez-Checa JC, Kaplowitz N.
Cell Death & Disease 2014 Jan; 5:e989.
Application:WB-Tr, Mouse, Primary mouse hepatocytes.
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JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death.
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