Western blot analysis of HAND2 over-expressed 293 cell line, cotransfected with HAND2 Validated Chimera RNAi ( Cat # H00009464-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HAND2 monoclonal antibody (M06), clone 3D5 (Cat # H00009464-M06 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq